Aligning Construct Strategy, Stability & Biology for Modern Structure-Based Drug Design
- Designing construct strategies that maximize structural yield and biological fidelity
- Balancing speed and exploration when screening multiple protein variants in parallel
- Demonstrating why high expression yield alone is insufficient, and how prioritising functional competence and conformational relevance have prevented misleading ligand poses and downstream design errors
- Delving into expression, production, solubilisation, and stabilisation strategies to overcome heterogeneity and preserve biologically relevant conformations