Resolving an Allosteric Binding Pocket in the HSV H-P Complex to Inform Antiviral Drug Design
- Optimizing Cryo-EM data acquisition and processing strategies to resolve ligand-bound states in conformationally heterogenous, multi-subunit viral complex
- Applying cryo-EM and complementary biophysical experiments to reveal ligand-stabilised allosteric binding sites that were absent in apo structures and not predictable from homology or co-folding models
- Recognizing limits of computational and biophysical methods, and utilizing mutational and omics data to validate binding sites